Characterization of anthocyanins from Camellia sinensis growing i | 58580
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Characterization of anthocyanins from Camellia sinensis growing in Kangra Valley by UPLC/Q-TOF-MS analysis and their stability studies

Joint Event on 9th World Congress on Chromatography & 24th International meet on Pharmaceutical Biotechnology

May 13-14, 2019 | Paris, France

Amita Kumari, Ashu Gulati and Sushil Kumar Maurya

CSIR-Institute of Himalayan Bioresource Technology, India

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Extraction of anthocyanins from purple tea shoots (Camellia sinensis) growing in Kangra valley, India was carried out using column chromatography. Characterization of anthocyanins was done by ultraperformance liquid chromatography-quadrupole-time of flight mass spectrometry. Fourteen (14) anthocyanins viz., pelargonidin-3- 5-di-glucoside, pelargonidin-3-sophoroside, pelargonidin-3- glucoside-5-glucoside, delphinidin-3-rutinoside, pelargonidin-3-rutinoside, cyanidin-3-5-O-di- glucoside, cyanidin-3-rhamnoside, pelargonidin-3-glucoside, cyanidin-3-rhamnoside, cyanidin-3-rutinoside, cyanidin-3-rutinoside-5-glucoside, delphinidin-3-glucoside, catechinpelargonidin-3-rutinoside dimer, cyanidin-3-glucoside, peonidin-3- rutinoside, peonidin-3-glucoside, peonidin-3-sophroside-5-glucoside were characterized on the basis of mass fragmentation pattern. The anthocyanins content ranged between 0.066±0.038 to 2.341±0.015% on dry weight in purple tea shoots. Catechin-rich fraction was extracted as a by-product from purple tea shoots and identified by HPLC. Intermolecular copigmentation was performed with catechin-rich extract and ascorbic acid for increasing the stability of anthocyanins. Catechin-rich extract showed higher stability of anthocyanins compared to ascorbic acid, which was investigated using UPLC-QTOF-MS. These copigmented anthocyanins can be used as a natural food colorant.

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