Journal of Chromatography & Separation Techniques
Open Access

Capillary zone electrophoresis as a tool for proteomics research

2^nd International Conference and Expo on Separation Techniques

September 26-28, 2016 Valencia, Spain

Norman J Dovichi

University of Notre Dame, USA

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has recently attracted attention as a tool for shotgun proteomics. However, its performance for this analysis has fallen far below that of reversed phase liquid chromatography (RPLC)-MS/MS. Here, we report the use of a CZE method with a wide separation window (up to 90 min) and high peak capacity (~300). This method is coupled to an Orbitrap fusion mass spectrometer via an electro-kinetically pumped sheath flow interface for analysis of complex proteome digests. Single-shot CZE-MS/MS identified over 10000 peptides and 2100 proteins from a HeLa cell proteome digest in ~100 min. This performance is nearly an order of magnitude superior to earlier CZE studies and is within a factor of 2 to 4 of state-of-the-art nano ultrahigh pressure LC system.

Biography :

Email: ndovichi@nd.edu