Aqueous normal phase chromatography: A new approach to complex sa | 54161
Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

+44 1300 500008

Aqueous normal phase chromatography: A new approach to complex sample analysis

World Congress on Chromatography

September 21-23, 2016 Amsterdam, Netherlands

Joseph J Pesek

San Jose State University, USA

Keynote: J Chromatogr Sep Tech

Abstract :

The analysis of metabolic, food, forensic, clinical, biological and physiological samples often encompasses complex matrices containing hundreds of compounds. The most comprehensive and useful approach for both targeted and untargeted analyses is LCMS. Reversed-phased HPLC provides a robust method for the separation of hydrophobic analytes but the available options for the separation of hydrophilic compounds are not as reliable. The most common approach currently in use, hydrophilic interaction liquid chromatography (HILIC) is often plagued by non-reproducible results, poor column lifetime and long equilibration times between gradient elution runs that are necessary for most complex samples. These problems can in most cases be avoided or significantly diminished by developing analytical protocols based on aqueous normal phase (ANP) chromatography using columns fabricated with silica hydride support materials. This presentation will illustrate the use of ANP for the analysis of polar compounds in a variety of applications. The chromatographic formats utilized have excellent intra-day, inter-day and column-to-column reproducibility, long column lifetimes and very short (1-2 column volumes) equilibration times. An additional advantage of the silica hydride-based columns is that they function in the reversed-phase as well as the normal-phase modes thus providing a global analysis strategy for multi-component samples.

Biography :

Joseph J Pesek received his BS degree in Chemistry from the University of Illinois, Urbana and his PhD in Analytical Chemistry from the University of California, Los Angeles. He did a one-year Post-doctoral fellowship at UCLA before becoming Assistant Professor of Chemistry at Northern Illinois University. He moved to San Jose State University becoming Professor of Chemistry and has also served as Department Chair, and Dean for Graduate Studies and Research. He has over 225 publications, 3 books, 4 patents and has made over 200 presentations at a variety of symposia and meetings. He is one of the Editors for the Journal of Separation Science.