Journal of Probiotics & Health
Open Access
ISSN: 2329-8901
ISSN: 2329-8901
Pina-Ronces Laura Gabriela, Guardado-Mendoza Rodolfo and Reyes-Escogido Lourdes
University of Guanajuato, Mexico
Posters-Accepted Abstracts: J Prob Health
Several Lactobacillus plantarum strains have been recognized due to the beneficial effect that may confer to health of the host. It has been reported that this strains have the ability to produce exopolysaccharides (EPS) which have been studied because of their antioxidant capacity. The aim of this assay was to determine the yield of EPS produced by twelve Lactobacillus plantarum strains growth on different carbon sources and to know the antioxidant activity of EPS obtained. The strains were grown in MRS broth with one of three carbon sources (Glucose, Lactose and Galactose). Crude EPS were extracted and quantified by anthrone method. Antioxidant activity for each EPS was determined by 2, 2-diphenyl-1-picrylhydrazyl free radical (DPPH) method, ascorbic acid was used as standard. All assays were performed in triplicate and results were expressed as mean�?±SD. The yields of EPS obtained in the three carbon sources were 2.17, 1.78 and 1.95 g/l for Glucose, Lactose and Galactose respectively without statistically difference. It means that the carbon source had not influence on the EPS yield. Although the antioxidant activity determined for EPS showed activities in a range from 32.75 to 76.02% being higher for EPS produced in medium with glucose (p=0.000). Antioxidant activity was similar to the ascorbic acid. The obtained results and knowing that the strains were isolated from humans, we could consider that possibly these strains play an important role in counteracting oxidative stress in vivo and their activity will be influenced by the diet of host.
Email: laura_pina06@hotmail.com