Matsushita Kenji1, Kawai Junya2, Sakuta Tetsuya1, Oyama Toru1, Miyashita Keiko1, Emoto Makiko1, Morimoto Yoko1 and Tokuda Masayuki1
1Kagoshima University Graduate School of Medical and Dental Sciences, Japan
2Hokuto Corporation, Japan
Posters-Accepted Abstracts: J Prob Health
We examined the antimicrobial activity of an extract of Hericium ernaceus toward cariogenic bacteria and periodontal diseaserelated bacteria in vitro and in the human oral cavity and we evaluated the possibility of the specimen as a new oral care agent. We prepared ethanol extracts of several mushrooms, Pleurotus eryngii, Grifola frondosa, Hypsizygus marmoreus, Hypsizygus marmoreus (white), H. erinaceus and Lentinula edodes. Then ethyl acetate-soluble extracts (Fr. L-9) of H. erinaceus. Porphyromonas gingivalis ATCC 33277, Streptococcus mutans MT 8148 and S. sobrinus 6715 were incubated with those mushroom extracts in broth and OD 595 nm in the cultures was measured. Healthy subjects (n=10) rinsed their mouths with a suspension of H. ernaceus extracts, cetylpyridinium chloride (CPC) and sterilized distilled water, respectively, and discharged liquids were collected at 0-5 hours after rinsing. Then bacteria in the liquids were measured by counting colony-forming units (CFU) and by quantitive polymerase chain reaction (qPCR). Extracts of H. erinaceus exhibited strong antimicrobial activity toward P. gingivalis. Fr.L-9 from H. erinaceus extracts also showed antimicrobial activities toward P. gingivalis, S. mutans, and S. sobrinus. CFU experiments showed that S. mutans and P. gingivalis were decreased by rinsing with H. erinaceus extracts. Results of qPCR analysis showed that rinsing with H. erinaceus extracts did not affect the total number of bacteria but decreased the percentage of P. gingivalis to total bacteria in the discharged liquids. Extracts of H. erinaceus have antimicrobial activities toward P. gingivalis and S. mutans and may improve the human oral microbiome from dysbiosis to synbiosis.
Matsushita Kenji has completed his PhD from Kagoshima University in Japan and Post-doctoral studies from Johns Hopkins University School of Medicine in USA. He is the Head of Department of Oral Disease Research at National Center for Geriatrics and Gerontology in Japan.
Email: [email protected]