Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
RuAngelie Edrada-Ebel
University of Strathclyde The John Arbuthnott Building, United Kingdom
Posters & Accepted Abstracts: J Chromatogr Sep Tech
High resolution Fourier transform mass spectrometry (HRFTMS) was employed as complimentary metabolomic tool to dereplicate chemical profiles of sponge-associated microbes. Sponges act as hosts to a diverse population of symbiotic organisms including bacteria and fungi. These symbionts offer benefits to the host including protection, nutritional benefit and support to the sponge skeleton. They have also been recognised as an important source of secondary metabolites which may have bioactivity and therefore medicinal potential. The innovative strategy involved targeted cultivation and isolation of biologically active compounds. Principal Component (PCA), Hierarchical Clustering (HCA), and Orthogonal Partial Least Square-Discriminant (OPLS-DA) analyses were used to evaluate HRFT mass spectral data of culture extracts. The results of the statistical analysis identified and validated the best culture conditions and extraction procedure which optimized the isolation of novel bioactive metabolites. Production of secondary metabolites were investigated in several of the bacterial symbionts that were isolated from marine sponges. Novel secondary metabolites were screened using high resolution mass spectrometrybased metabolomics approaches. Metabolomic profiling using HR-ESIFTMS-MS fragmentation were done at different stages of the growth phase for both solid and liquid culture media and subjected to molecular networking. Molecular networking the mass fragmentation data pinpoints functionalities that can be correlated to bioactivity and fermentation parameters as well as to predict and enhance the biosynthetic pathway involved in the production of the target secondary metabolite.