Journal of Chromatography & Separation Techniques
Open Access

Departmentof Crop Physiology, Jiangxi Agricultural University, jiangxi Province, China

Biography

Dr. Xiaoping Huang is affiliated to Departmentof Crop Physiology, Jiangxi Agricultural University, jiangxi Province, China, where Dr. Xiaoping Huang is currently working as  Professor . Dr. Xiaoping Huang has numerous publications within the specialty and published in reputed national and international peer-reviewed journals. Dr. Xiaoping Huang is actively associated with different national and international societies and academies.

Publications

• Research Article   
  Evaluation of Six Protocols for Protein Extraction from Rice Young Panicles by Two-Dimensional Electrophoresis
  Author(s): Xiaoping Huang* and Zhou H
  
  ABSTRACT For most reported proteomics approaches, protein extraction and sample preparation are of crucial importance for optimal results. However, extraction of protein from crop plant tissues remains a great challenge due to low protein content and abundant secondary metabolites that prominently interfere with isoelectric focusing and subsequent proteomic analysis. Up until now, no attempts are focused on comparison of protein extraction procedures from rice young panicles. To establish a high-efficiency protein extraction protocol suitable for two-dimensional electrophoresis (2-DE) in rice young panicles, six protocols for protein preparation were evaluated: (1) Phenol extraction; (2) Mg/ Nonidet P-40 (NP-40) extraction; (3) Tris-Base/acetone precipitation; (4) SDS extraction; (5) trichloroacetic acid (TCA)/acetone/phenol extraction; (6) TCA/acetone precipitation. The stu.. View More»
  DOI: 10.35248/2157-7064.21.12.438

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