ISSN: 2157-7064
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Department of Chemical and Biomolecular Engineering, University of National Singapore, Queenstown, Singapore
Research Article
Attempted Fractionation of LB Lennox Medium via Reversed Phase High Performance Liquid Chromatography
Author(s): Wenfa Ng*
Mass balance analysis is a highly useful tool for chemical engineering analysis of biological processes. Specifically, composition and amounts of inputs to a cell could be correlated with measurement of metabolic by-products and outputs for inferring metabolic fluxes flowing through specific cellular metabolic pathways. However, the composition of many common microbiological growth medium remains ill-defined with batch-to-batch variation. Thus, a need exists for developing methods for effective fractionation and separation of common growth medium. Using 5 g/L yeast extract, 10 g/L tryptone, and LB Lennox medium as model systems, this work attempted the fractionation of the three complex growth mixtures using C-18 Reversed Phase High Performance Liquid Chromatography (RPHPLC). Results revealed no effective fractionation of the three mixtures. More importantly, experiment results indica.. View More»
DOI:
10.35248/2157-7064.22.13.484