Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Vishwakarma J

Dept. of Clinical Pharmacology, TN Medical College & BYL Nair Hospital, Dr. AL Nair Road, Mumbai Central, Mumbai 400 008, Maharashtra, India

  • Research Article   
    Application of High-Performance Thin Layer Chromatography in the Quantitative Estimation of Serum Clobazam Levels
    Author(s): Kulkarni-Munshi R* and Vishwakarma J

    This study was aimed to develop and validate a High-Performance Thin Layer Chromatography (HPTLC) method for the quantitative determination of serum clobazam levels. This simple and sensitive method will help in therapeutic drug monitoring that aids in the clinical management of patient therapy. Chromatographic separation was carried out on a silica gel 60F254 HPTLC plate using a mixture of Toluene:Methanol:Glacial acetic acid (15:1:0.16, v/v/v) as the mobile phase. Densitometric detection was carried out at 231 nanometers. The method was validated for specificity, precision, accuracy, robustness, linearity, limit of detection, and limit of quantification. Linear .. View More»
    DOI: 10.35248/2157-7064.20.11.433

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