Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Sonal Desai

Department of Pharmaceutical Quality Assurance, SSR College of Pharmacy, Silvassa, Dadra and Nagar Haveli, India

  • Research Article   
    Factorial Design Based HPLC-PDA Method Optimization for Quantification Of Hesperidin in Orange Peel
    Author(s): Priyal Desai, Niyatee Thakor and Sonal Desai*

    Background: HPLC-PDA method was developed using factorial design approach and validated for the estimation of hesperidin in orange peel powder. A 32 factorial design was applied to evaluate the effect of the two factors that are mobile phase composition and flow rate on the various chromatographic responses such as retention time, area, and tailing factor. Chromatographic separation was achieved using Sunniest ECO (250 mm × 4.6 mm, 5 μm) column as a stationary phase and optimized mobile phase consisted of Methanol: water in ratio of 51.5:48.5 v/v at a flow rate of 1.1 mL/min. Detection wavelength was 285 nm. The retention time of hesperidin was found to be 4.01 min. Results: In validation studies, calibration curve was found to be linear over the range of 0.2-6 μg/mL with the regression coefficient of 0.996. The LOD and LOQ were f.. Read More»
    DOI: 10.35248/2157-7064.23.14.494

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