Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Department of Pharmacognosy, L. M. College of Pharmacy, Ahmedabad, India
Research Article
Determination of Flavonoids in Stem Bark of Manilkara hexandra by Simultaneous and Validated HPLC Methods
Author(s): Sapna Sharma, Sarojkumar Katara and Mamta B. Shah*
Manilkara hexandra Roxb. Dubard, another name is Mimusops hexandra is a tall, glabrous evergreen tree belonging to the Sapotaceae family. Ethnopharmacological uses of M. hexandra in toothache, tonic, dysentery, diarrhea, alimentary disorders, infertility and veterinary use by tribal people of different regions in India. Research articles revealed immunostimulant, inhibitory effect on the SARS-CoV-2 protease enzyme, antiulcer and antioxidant activity of stem bark. A simple, accurate and reproducible reversed-phase liquid chromatographic method was developed for the qualitative and quantitative determination of four bioactive flavonoids (quercetin, luteolin, kaempferol and apigenin) from the stem bark extract of M. hexandra. Chromatographic separation was performed on a C18 column (5 μm C18, 4.6 × 250 mm) with a mobile phase consisting of 0.5.. View More»