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Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

+44 1300 500008

Sanjay Kumar Sharma

Amol Pharmaceuticals Private Limited, Jaipur, Rajasthan, India

Publications
  • Research Article   
    Validated UPLC Method for Identification and Quantification of Phenazopyridine Hydrochloride Drug: A Green Analytical Technique
    Author(s): Omprakash Saini*, Sanjay Kumar Sharma and Yogesh Sharma

    Green Chemistry talks about any environmental technique or practice which may reduce waste, materials, hazards, risk, energy, environmental impact, and operational cost, also increase productivity. Ultra Performance Liquid Chromatography (UPLC) is a modernized technique of High-performance Liquid chromatography (HPLC), which is a Green Technique in the true sense. To enhance those practices, a simple, sensitive, accurate, selective, and precise Ultra Performance Liquid Chromatography (UPLC) method was developed for the identification and quantification of the Phenazopyridine Hydrochloride Drug. The separation was performed on a BEH C18-column (1.7 µm, 2.1 mm × 50 mm) using a mobile phase 20 mM ammonium acetate buffer as mobile phase A and 100% Acetonitrile as a mobile phase-B in gradient elution. The detection was performed at a fixed wavelength (λ=280 nm), with a f.. View More»
    DOI: 10.35248/2157-7064.22.S1.002

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