Journal of Chromatography & Separation Techniques
Open Access

Department of Analytical Chemistry, University of Modern Science and Arts, 6th of October City, Egypt

Publications

• Research Article   
  Validated Chromatographic Methods for Determination of Pioglitazone and Glimepiride in Pharmaceutical Dosage Form and in Human Plasma
  Author(s): Safaa M. Riad, Miriam Farid, Mona H.Abdel Rahman and Dina Atef*
  
  Two simple, sensitive and selective chromatographic methods were described for simultaneous determination of Pioglitazone (PGZ) and Glimepiride (GLM) in their tablet formulation and in human plasma. The first method Thin Layer Chromatography (TLC) was performed on aluminium TLC plates pre coated with silica gel 60 F254 as the stationary phase and a mixture of Toluene: Methanol: Ethyl acetate as the mobile phase by ratio 6:2:4, v/v/v to give compact spots for PGZ at Rf=0.88 and GLM at Rf=0.63 with linearity range and mean percentage recoveries 0.5-5 μg spot-1, 99.00% for PGZ and 0.7-2 μg spot-1, 99.97% for GLM and the chromatogram was scanned at 266 nm. The second method High Performance Liquid Chromatography (HPLC) was developed for determination of the co-administered drug Duloxetine (DUL) with the drugs of interest (PGZ and GLM). Using Xterra C18 column and gradient mobile pha.. View More»
  DOI: 10.35248/2157-7064.22.S1.004

  Abstract HTML PDF