Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Omerdic EO

Development and Registration Department, Bosnalijek, 71000 Sarajevo, Bosnia and Herzegovina

  • Research Article   
    Simultaneous Estimation of Four Preservatives in Pharmaceutical Ointment by RP-HPLC
    Author(s): Alagic-Dzambic L*, Dacic M, Stanic M, Omerdic EO and Dzambic M

    A simple, specific, precise and accurate reverse phase HPLC method has been developed for the simultaneous determination of preservatives (Methyl Para hydroxybenzoate, Ethyl para hydroxybenzoate, Propyl para hydroxybenzoate and Chlorocresol) in ointment. The chromatographic separation was achieved on Zorbax column, 150 mm × 4.6 mm, 5 µm, using PDA detector. The mobile phase was 50% Methanol (v:v), at a flow rate of 1.0 mL/min. All preservatives were detected at 230 nm, at 30ºC temperature for column. The retention times were 2.15 min for Methyl para hydroxybenzoate, 2.70 min for Ethyl para hydroxybenzoate, 3.69 min for Propyl para hydroxybenzoate and 4.01 min for Chlorocresol, with incredibly good resolution between peaks. The method was validated accordin.. View More»
    DOI: 10.35248/2157-7064.20.11.434

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