Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Mustafa A

Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan

  • Research Article   
    Simultaneous Determination of Amoxicillin, Clarithromycin and Esomeprazole in Mice Plasma after Oral Administration by Reverse Phase HPLC Method
    Author(s): Mustafa A, Ali H*, Bibi R, Hassan A, Khan S and Khan SA

    A simple and precise reverse phase high-performance liquid chromatography (RP-HPLC) method for simultaneous determination of amoxicillin, clarithromycin and esomeprazole in mice plasma after an oral administration was developed and validated. An isocratic elution was achieved on C18 column with a mobile phase containing buffer, potassium di hydrogen phosphate (KH2PO4 ) 0.05 M at pH 5 and methanol (60:40 v/v) at a flow rate of 1 mL/min, injection volume was 10 µL and UV detection was kept at 205 nm. Linearity was in the range of 0.5-100 µg/mL for amoxicillin, 50-1000 µg/mL for clarithromycin and 0.1-100 µg/mL for esomeprazole. Limit of detection (LOD) and Limit of quantification (LOQ) were 0.26 µg/mL and 0.79 µg/mL for amoxicillin, 8.97 µg/mL and 27.20 µg/mL for clarithromycin, 0.13 µg/mL and 0.39 µg/mL for esomeprazole respec.. Read More»
    DOI: 10.35248/2157-7064.21.12.439

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