Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Personalized Medication Management Unit, Children’s Cancer Hospital Egypt, Zeinhom, Cairo, Egypt
Research Article
Developing a Ultra-High-Performance Liquid Chromatographic-Diode Array Method to Quantify Voriconazole in Human Plasma
Author(s): Yousra elmazahy*, Ahmed Farag, Lobna shalaby and Mohamed Nagy
A fast and selective Reversed-Phase (RP) liquid chromatography-Diode Array (DAD) method was developed and validated to determine Voriconazole in human plasma. Thanks to the high throughput and sensitivity of UltraHigh-Performance Liquid Chromatographic (UHPLC) Chromatography, there were only 7.5 minutes of run time, and no buffers were used. 200 μl serum and 200 μl internal standard fluconazole were added. After simple protein precipitation with 400 μl methanol, 10 μl of the clear supernatant separated on Agilent Zorbax Eclipse Plus® C18 Column (50*2.1 mm, 1.8 μm). The column temperature was maintained at 40°C. The flow rate was 0.4 ml/min using a gradient elution of methanol and water. The retention time of Fluconazole and Voriconazole were 4.2 and 6.2 min, respectively. Detection was performed using a diode array detector set at a wavelength of 261 nm. The me.. View More»
DOI:
10.35248/2157-7064.23.14.516