Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Department of Pharmaceutical Chemistry, KLE College of Pharmacy Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India
Research Article
Development and Validation of HPTLC Method for the Simultaneous Estimation of Curcumin and Azadirachtin
Author(s): Sushmita I. Hiremath, Mahesh. S. Palled*, Rajesh K Joshi, Mahendra Kumar Chouhan and Shailendra S. Suryawanshi
Objective: The objective of present work is to develop and validate HPTLC method for simultaneous estimation of Curcumin and Azadirachtin in marketed formulation
Materials and methods: HPTLC method was developed using a solvent system Toluene: Ethyl acetate: Ammonia: Formic acid (4:3:2.5:0.5 v/v/v/v) using a stationary phase Silica Gel 60 F254 and the saturation time is 15 min. The developed method was standardized in terms of validation parameters such as specificity, linear range, precision, robustness, ruggedness and reproducibility as per ICH guidelines. Newly developed and validated method was successfully applied for estimation of Curcumin and Azadirachtin in marketed formulation.
Results: The linearity range for the both Curcumin and Azadirachtin was found to be 1.5-15 μl/spot. The limit of detection foun.. View More»
DOI:
10.35248/2157-7064.22.13.483