Journal of Clinical Chemistry and Laboratory Medicine
Open Access

GEBISA, Research Foundation, Perugia, Italy

Biography

Prof. Gian Carlo Di Renzo is currently Professor and Chair at the University of Perugia (2004 -), Director of the Reproductive and Perinatal Medicine Center (1996 -), former Director of the Midwifery School (2004-2016), University of Perugia, Director of the Permanent International and European School of Perinatal and Reproductive Medicine (PREIS) in Florence (2012 – ).

Publications

• Research Article   
  Validation of Immunoblot SDS-page as a Qualitative and a Quantitative Method for Determination of Urinary Cystatin C in Neonates
  Author(s): Chiara Grasselli*, A. Barbati, L. Cesarini, R. Pellegrino and G.C. Di Renzo
  
  Objective: The measurement of urinary Cystatin C (cyst-C) can be a very good way for early identifying newborns predisposed to renal function impairment. PETIA, PENIA and EIA are the immunometric methods used for quantitative determination of cyst-C in human biologic fluid but they have some interference and do not allow realizing qualitative analysis. The present study performs a validation of Immunoblot SDS-PAGE for the analysis of urinary cyst-C. Methods: urines were collected from neonates of the nursey at S. Maria della Misericordia Hospital. Urinary cyst-C was investigated by the SDS-PAGE electrophoresis, the immunoblot and the reading of optical density. Results: the qualitative analysis showed two different molecular forms: a reactivity at about 70 KDa in all samples and a reactivity a.. View More»
  DOI: 10.35248/jcclm.20.3.141

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