Journal of Clinical Chemistry and Laboratory Medicine
Open Access
ISSN: 2736-6588
ISSN: 2736-6588
Department of Surgical and Biomedical Sciences, Section of Obstetrics and gynecology, University of Perugia, Perugia, Italy
Chiara Grasselli completed doctoral program in translational and molecular medicine- DIMET, from department of medicine and surgery (School of medicine and surgery). Publications include: 36-kDa Annexin A3 Isoform Negatively Modulates Lipid Storage in Clear Cell Renal Cell Carcinoma Cells, PKHhigh / CD133 + / CD24- Renal Stem-Like Cells Isolated from Human Nephrospheres Exhibit In Vitro Multipotency.
Research Article
Validation of Immunoblot SDS-page as a Qualitative and a Quantitative Method
for Determination of Urinary Cystatin C in Neonates
Author(s): Chiara Grasselli*, A. Barbati, L. Cesarini, R. Pellegrino and G.C. Di Renzo
Objective: The measurement of urinary Cystatin C (cyst-C) can be a very good way for early identifying newborns
predisposed to renal function impairment. PETIA, PENIA and EIA are the immunometric methods used for
quantitative determination of cyst-C in human biologic fluid but they have some interference and do not allow
realizing qualitative analysis. The present study performs a validation of Immunoblot SDS-PAGE for the analysis of
urinary cyst-C.
Methods: urines were collected from neonates of the nursey at S. Maria della Misericordia Hospital. Urinary cyst-C
was investigated by the SDS-PAGE electrophoresis, the immunoblot and the reading of optical density.
Results: the qualitative analysis showed two different molecular forms: a reactivity at about 70 KDa in all samples and
a reactivity a.. View More»
DOI:
10.35248/jcclm.20.3.141