Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

Divya Vilas K

Department of Pharmaceutical analysis, KLR Pharmacy college, Palwancha, Telangana, India

  • Research Article   
    A Rapid LC-MS/MS Method for Quantification of Acetaminophen in Human Whole Blood
    Author(s): Divya Vilas K, Sandeep Reddy C, Prashanthi R and Ankit C*

    Acetaminophen (paracetamol, N-(4-hydroxyphenyl) acetamide) is one of the most prescribed drugs for the pain management, minor aches, and treatment of fever. Quantification of acetaminophen in pre-term and term neonates and small children requires the availability of highly sensitive assays in small volume blood samples. We developed and validated an LC-MS/MS assay for the quantification of acetaminophen in human whole blood. The drug molecule was extracted from whole blood samples followed by protein precipitation extraction technique followed by LC-MS/MS analysis. An excellent analyte separation was achieved by using a phenomenex Gemini® C18 column (50× 3.0 mm, 3 μm) using mobile phase A-Water:Formic Acid (100:0.1) and Acetonitrile:Formic acid (100:0.1) as mobile phase B. With 3.40 minutes as analytical run time, this analytical method demonstrated linearity between 50.0.. View More»
    DOI: 10.35248/2157-7064.20.11.432

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