Journal of Clinical Chemistry and Laboratory Medicine
Open Access
ISSN: 2736-6588
ISSN: 2736-6588
Department of Pathology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning Province,110042, China
Research Article
The Effect of Immunomodulator Thymosin α1 on the Expression of TNBC PD-L1 Through Wnt/β-Catenin Signaling Pathway
Author(s): Zheng Jian*, Li Xiaoxi, Jiang Youhong and Cai Cunwei
Purpose: Explore whether thymosin affects the expression of PD-L1 on the surface of tumor cells, which in turn
affects the immunotherapy of triple-negative breast cancer.
Methods: After treating human breast cancer MDA-MB-231 cells with different concentrations of thymosin α1, the PD-L1 expression of the cells was tested for protein and gene. Through bioinformatics analysis, the target gene was screened out, and the signal pathway that thymosin α1 might participate in was further verified.
R After adding 130 μM and 160 μM of thymosin α1, the expression of PD-L1 in MDA-MB-231 cells was inhibited at 12 h, 24 h, 48 h and 96 h. The related pathways of genes down-regulated by Tα1 in KEGG are: Cancer pathway, basal cell epithelioma, apoptosis, Hippo and Wnt signaling pathway; immune pathway, such .. View More»
DOI:
10.35248/JCCLM.22.05.223