Mycobacterial Diseases
Open Access
ISSN: 2161-1068
ISSN: 2161-1068
Department of Mycobacteriology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Research Article
Comparison of Real-Time PCR, RFLP-PCR and Multiplex PCR with GeneXpert MTB/RIF for Diagnostic Rifampicin Resistance among Pulmonary Tuberculosis
Author(s): Parisa Farnia, Alireza Javadi*, Shima Seif, Poopak Farnia and Jalaledin Ghanavi
Background: Rapid diagnosis of TB globally remains challenging and emergence of resistant strains represents a significant threat. Improved techniques on diagnosis of tuberculosis is a major concerning for this burden control and is crucial for new tuberculosis control strategy. This study aimed to evaluate diagnostic accuracy of GeneXpert MTB/RIF assay for confirming rifampicin resistance among those suspects comparing to multiplex PCR, RFLP-PCR and real-time PCR amplifications.
Methods: Sputum samples were collected and analysed during 2021 to 2023. Sputum was transferred to the bacteriology laboratory for MTB/RIF detection using conventional bacteriological and molecular based-assays. Sensitivity and specificity were accounted for the stained smear and other molecular methods according to GeneXpert MTB/RIF as the gold standard.
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