Journal of Clinical & Experimental Dermatology Research
Open Access
ISSN: 2155-9554
+44 1478 350008
ISSN: 2155-9554
+44 1478 350008
Xianling Cong
China-Japan Union, Hospital of Jilin University,
Changchun 130033
China
Research Article
Rapid Detection of Sporotrichosis by ABC-ELISA
Author(s): Yaqin Zhang, Jianjiao Zu, Venkatesh Pyla, Qing Yue and Xianling CongYaqin Zhang, Jianjiao Zu, Venkatesh Pyla, Qing Yue and Xianling Cong
Background: Avidin-biotin-horseradish peroxidase enzyme complex, enzyme-linked immunosorbent assay (ABCELISA) is widely used in detecting the soluble bacterial, viral antigen and antibody, but the reports of detecting mycotic antigens were few .We used the method to detect Sporothrix schenckii pathogen of sporotrichosis in smear. Aim: To provide a rapid diagnostic method for sporotrichosis. Methods: 20 cases of sporotrichosis-in doubt we scraped pus or tissue fluid from the skin lesion and perform ABCELISA by reagent vectastatin ABC kit, rabbit anti Sporothrix schenckii serum. Results: 18 of 20 cases were diagnosed as sporotrichosis. 14 of 18 cases were positive (77.8%) by ABC-ELISA stain, 5 of 18 cases were positive (27.7%) by Gram stain. Conclusion: ABC ELIS.. View More»
DOI:
10.4172/2155-9554.1000162