ISSN: 2155-9554
+44 1478 350008
Saroj Suvanasuthi
Thailand
Research Article
In vitro Culture and Histological Characterization of Extracted Human Hair Follicles
Author(s): Rattapon Thuangtong, Pitchaya Maneeprasopchoke, Chatchawan Srisawat, Chinnavuth Vatanashevanopakorn, Suchanan Hanamornroongruang, Sarawut Junnu and Saroj Suvanasuthi
Rattapon Thuangtong, Pitchaya Maneeprasopchoke, Chatchawan Srisawat, Chinnavuth Vatanashevanopakorn, Suchanan Hanamornroongruang, Sarawut Junnu and Saroj Suvanasuthi
Background: In vitro-cultured hair, follicles can serve as an excellent model system for exploring the hair follicle biology as well as drug delivery targets. In this study, we aimed to investigate how to maintain whole human hair follicles in vitro and histologically characterize the hair follicles thereof.
Methods: Donated hair follicle specimens from hair transplant operation were cultured in vitro using a medium consisting of Williams` medium E, L-glutamine, insulin, hydrocortisone, penicillin, streptomycin, and amphotericin B. The growth of hair follicles was examined daily using a digital microscope. Their histological features were studied at day 0, 7, and 14 to characterize the changes of hair follicles maintained in culture.
Results: Cultured hair follicles continued to grow at a.. View More»
DOI:
10.4172/2155-9554.1000182