Journal of Clinical & Experimental Dermatology Research
Open Access
ISSN: 2155-9554
+44 1478 350008
ISSN: 2155-9554
+44 1478 350008
Min Chen
Laboratory of Molecular Biology for Skin Disease and STIs, Institute of Dermatology, 12 Jiangwangmiao Street, Nanjing, Jiangsu,
China
Research Article
The Special DNA Methylation Status in the Promoter of IL-12B in Psoriatic Epidermis
Author(s): Ningyan Gu, Chao Luan, Xingfeng Wu, Yan Wang, Mingjun Jiang, Min Chen and Pangeng CuiNingyan Gu, Chao Luan, Xingfeng Wu, Yan Wang, Mingjun Jiang, Min Chen and Pangeng Cui
Background: It is unclear the metylation status of CpG islands within interleukin-12B (IL-12B) in psoriasis, although increasing evidence indicates that the abnormal DNA methylation is involved in the pathogenesis of psoriasis.
Objective: We investigated the methylation status of IL-12B gene in psoriatic epidermis.
Methods: The DNA specimens were obtained from the involved and uninvolved epidermis of 30 patients with plaque psoriasis and 28 healthy persons as the control group. Methylation of 26 CpG sites in the promoter within IL-12B gene was examined by bisulfite sequencing. The severity of disease was evaluated by psoriasis area and severity index (PASI).
Results: The mean methylation rate in the promoter of IL-12B in involved psoriatic epidermis, uninvolved psoriatic epidermis and hea.. View More»
DOI:
10.4172/2155-9554.1000341