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Factors Affecting the Concentration of Diphenylmethane-4,4′-Diisocyanate in Freund’s Complete Adjuvant. Can They Affect the Outcome of the Guinea-Pig Maximization Test?
Author(s): Haneen Hamada, Magnus Bruze, Erik Zimerson, Marléne Isaksson and Malin EngfeldtHaneen Hamada, Magnus Bruze, Erik Zimerson, Marléne Isaksson and Malin Engfeldt
Background: The animal assay Guinea Pig Maximization Test (GPMT) uses Freund’s complete adjuvant (FCA) to enhance the dermal sensitization in the animals. In a previous study, the degree of sensitization when investigating diphenylmethane-4,4′-diisocyanate (4,4′-MDI) varied. 4,4′-MDI is known to be a reactive diisocyanate and concern was risen that its instability and reaction with FCA might affect the outcome. Objectives: To investigate the stability of 4,4′-MDI and its possible reaction with components in FCA. Methods: The same preparations as used in the GPMT were prepared, i.e. 1.0% w/v 4,4ʹ-MDI in FCA/paraffin oil (40/60 v/v) and stored under different conditions for 48 hours. The content of 4,4′-MDI in both FCA/paraffin oil mixtures as well as in samples of pure substance were.. Read More»