Journal of Cell Signaling
Open Access
ISSN: 2576-1471
+44 1223 790975
ISSN: 2576-1471
+44 1223 790975
Joanne M. Dobson
New Zealand
Research Article
Induced Physical Distension of Rat Mammary Glands Accelerates the Onset of Apoptosis and Involution
Author(s): Claire V. C. Phyn1, Stephen R. Davis, Joanne M. Dobson, Kerst Stelwagen and Kuljeet SinghClaire V. C. Phyn1, Stephen R. Davis, Joanne M. Dobson, Kerst Stelwagen and Kuljeet Singh
Objective: Weaning is a process that results in a reduction in milk secretion, an increase in mammary epithelial cell (MEC) apoptosis, and involution of the mammary gland. The local mechanisms initiating MEC apoptosis and involution are unclear, although the physical morphology of the MEC may influence cell-cell and cell-extracellular matrix communication and thus may alter function. This study examined the effect of physical distension of alveoli on the early molecular events that occur at the onset of involution of rat mammary glands.
Methods: Mammary tissue was collected post-mortem from lactating Sprague-Dawley rats at 0, 1, 3, and 6 h (n=6 per time point) following acute physical distension of an inguinal gland with isosmotic sucrose solution (0.8 ml; equivalent to ~6 h worth of milk accumulation) via the teat canal followed by.. View More»
DOI:
10.4172/2576-1471.1000160