Chemotherapy: Open Access
Open Access
ISSN: 2167-7700
+44 1223 790975
ISSN: 2167-7700
+44 1223 790975
Hussain R Al-Turaifi
Institute of Cellular Medicine,
Newcastle upon Tyne, NE2 4HH
Research Article
Expression of Classical Embryonic Stem Cell Markers in Pancreatic Cancer Cells
Author(s): Hussain R Al-TuraifiHussain R Al-Turaifi
Background: Pancreatic cancer has been the third leading cause of cancer-related death in USA. Most of the cancer patients diagnosed in late stage which minimize the effectiveness of surgical intervention to less than 20 percentage. Moreover, chemo-radio therapy is not curative. Presence of Cancer Stem Cells (CSCs) within pancreatic tumor was reported by several groups using nonspecific biomarkers. Pluripotent transcription factors such as OCT4, SOX2 and NANOG, which are upregulated in embryonic stem cells in contrast to somatic cells, were detected in various type of cancer tumours from adult patients.
The aims of this study were to investigate the expression of the classical stem cell markers in Pancreatic Adenocarcinoma Cell line (PANC1).
Methods: PANC1 cells were characterised by RT-PCR/immuno-staining. Transient over-expression of s.. View More»