Journal of Clinical & Experimental Dermatology Research
Open Access
ISSN: 2155-9554
+44 1478 350008
ISSN: 2155-9554
+44 1478 350008
Djaffar Ould-Ali
Service de chirurgie plastique et réparatrice, Hôpital La Conception,
APHM, 180 Boulevard Baille 13385 Marseille cedex 05
France
Research Article
Bleomycin-induced Scleroderma in Nude Mice can be Reversed by Injection of Adipose Tissue: Evidence for a Novel Therapeutic Intervention in Systemic Sclerosis
Author(s): Djaffar Ould-Ali, Aurelie Hautier, Lucile Andrac-Meyer, Nathalie Bardin, Guy Magalon and Brigitte GranelDjaffar Ould-Ali, Aurelie Hautier, Lucile Andrac-Meyer, Nathalie Bardin, Guy Magalon and Brigitte Granel
Objective: Systemic sclerosis is an autoimmune disease characterised by uncontrolled fibrosis and vascular insufficiency of the skin and internal organs, without efficient treatments. Subcutaneous adipose transplants have been shown to exert trophic effects on surrounding tissue. We aimed to determine the effects of human adipose tissue in a murine model of sclerotic skin. Methods: Scleroderma was induced in 48 nude mice by daily subcutaneous injection of bleomycin during four weeks. Immediately after the final bleomycin injection, human subcutaneous adipose tissue was implanted into the subcutaneous space of mice using either the Coleman method or the micro-injection method. Epidermal and dermal thicknesses were assessed on skin biopsy specimens six weeks after fat implantation. Capillary density was assessed using immunohistochemistry with an .. View More»
DOI:
10.4172/2155-9554.1000164