Rheumatology: Current Research
Open Access

Charles J Malemud

Publications

• Research Article
  Monosodium Urate and Tumor Necrosis Factor-α Increase Apoptosis in Human Chondrocyte Cultures
  Author(s): Charles J Malemud, Yan Sun, Eric Pearlman, Nell M Ginley, Amad Awadallah, Bradley A Wisler and James E Dennis Charles J Malemud, Yan Sun, Eric Pearlman, Nell M Ginley, Amad Awadallah, Bradley A Wisler and James E Dennis
  
  Monosodium urate and tumor necrosis factor-α, are two potent mediators of separate inflammatory response pathways in arthritic joints where inflammation may be accompanied by the loss of chondrocyte vitality via apoptosis. To address this possibility in vitro, chondrocyte cultures were employed to determine the extent to which monosodium urate and recombinant TNF-α altered the frequency of apoptotic chondrocytes. Apoptosis as a function of the activation of p38 kinase, C-Jun-terminal kinase, signal transducer and activator of transcription-3 and/or the activity of xanthine oxidase was also studied. Using normal human chondrocytes, monosodium urate or recombinant tumor necrosis factor-α increased the frequency of apoptosis and activity of xanthine oxidase. However, the xanthine oxidase-specific inhibitor, febuxostat, failed to blunt this response. Monosodium urate, tu.. View More»
  DOI: 10.4172/2161-1149.1000113

  Abstract PDF

Relevant Topics