Journal of Clinical & Experimental Dermatology Research
Open Access
ISSN: 2155-9554
+44 1478 350008
ISSN: 2155-9554
+44 1478 350008
Catharina M. Lerche
Copenhagen University Hospital, Bispebjerg, Department of Dermatology,
D92, Bispebjerg Bakke 23, DK-2400 Copenhagen NV
Denmark
Letter to Editor
Topical Nutlin-3 Potentiates the UVB-induced p53 Response and Reduces DNA Photodamage and Apoptosis in Mouse Epidermal Keratinocytes in Vivo
Author(s): Catharina M. Lerche, Birthe Mørch Thomsen, Hans Christian Wulf and Robert GniadeckiCatharina M. Lerche, Birthe Mørch Thomsen, Hans Christian Wulf and Robert Gniadecki
Background/Aims: (-)-Nutlin-3 (nutlin) is a cis-imidazoline analog, which activates p53 by antagonizing murine double minute (Mdm2) protein. To our knowledge, no studies have assessed the effect of topical nutlin on cyclobutane pyrimidine dimers (CPD) repair and apoptosis. We therefore conducted a study in hairless mice to investigate the effect of topical nutlin on murine epidermis after UVB-radiation. Methods: Female C3.Cg/TifBomTac immunocompetent mice were treated with 100 μl 43 mM nutlin 30 min before and after irradiation with 3 SED (100 mJ/cm2) UVB. Animals were euthanized 24 hours after irradiation, the dorsal, treated skin was biopsied and fixed in 4% formalin. Sections were incubated with the antibodies against p53, thymine dimers and terminal deoxynucleotidyl transferase-mediated dUTP nick and labelling (TUNEL)... View More»
DOI:
10.4172/2155-9554.1000106