Rheumatology: Current Research
Open Access
ISSN: 2161-1149 (Printed)
+44-20-4587-4809
ISSN: 2161-1149 (Printed)
+44-20-4587-4809
Buthaina Al-Hudar
Syria
Research Article
HLADRB1 Gene is Highly Mutated in Omani Patients Affected with Rheumatoid Arthritis
Author(s): Buthaina Al-Hudar, Aziza Al-Busaidi, Ishita Gupta, Batool Hassan and Yahya TamimiButhaina Al-Hudar, Aziza Al-Busaidi, Ishita Gupta, Batool Hassan and Yahya Tamimi
Objectives: Rheumatoid Arthritis (RA) is a multifactorial autoimmune disease affecting synovial joints, surrounding tissues, and many other organs of the human body. HLA-DRB1 has a major role in the immune system by presenting peptides derived from extracellular proteins on antigen presenting cells and has been associated with RA. In this study, we screened for HLA-DRB1 mutations in Omani patients affected with RA.
Methods: Thirty blood samples from affected patients were examined in parallel with fourteen healthy control samples. HLA-DRB1 mutational status was examined using polymerase chain reaction (PCR) and sequencing.
Results: A total of 75 aberrations were identified in HLA-DRB1, of which 20% were polymorphisms. From the reported aberrations, 25.5% were silent mutations. Within exon-2, three non-synonymous mu.. View More»
DOI:
10.4172/2161-1149.1000170