Journal of Clinical and Cellular Immunology

Journal of Clinical and Cellular Immunology
Open Access

ISSN: 2155-9899

+44 1223 790975

Ashok Kumar

Ashok Kumar
Research Institute, Children’s Hospital of Eastern Ontario,
401 Smyth Road, Ottawa, Ontario, K1H 8L1

  • Research Article
    Characterization of In vitro Generated Human Polarized Macrophages
    Author(s): Salma Iqbal and Ashok KumarSalma Iqbal and Ashok Kumar

    Objective: Contact with invading pathogens and/or tissue injury leads to the polarization of macrophages into either a M1 or a M2 state which is further divided into M2a, M2b and M2c subsets. The human macrophage subsets have been poorly characterized. The present study was undertaken to characterize macrophage polarization using a non-exhaustive panel of surface markers with respect to M1, M2a, M2b and M2c macrophages and production of pro- and anti-inflammatory cytokines in response to various toll-like receptors (TLR), ligands.Methods: We generated various macrophage subsets by treating monocyte-derived macrophages (MDMs) with IFNγ (M1), IL-4 (M2a), LPS and IL-1β (M2b) or IL-10 (M2c) followed by stimulation with toll-like receptor (TLR)- 2, TLR-3 and TLR-4 agonists and analysis of surface marker and cytokines expression was carried .. View More»
    DOI: 10.4172/2155-9899.1000380

    Abstract PDF

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