Butylatedhydroxytoluene (BHT) and 4-methoxyphenol (4-MP) are phenol derivatives that are generally known for their antioxidant properties and depigmenting activities. The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance of BHT and 4-MP using gas chromatography-mass spectrometry (GC-MS). BHT and 4-MP samples were divided into two parts: control and treated. The control group remained untreated while the treated group was subjected to Mr. Trivedi’s biofield treatment. Control and treated samples were characterized using GC-MS. The GC-MS data revealed that the isotopic abundance ratio of 13C/12C or 2H/1H (PM+1/PM) and 18O/16O (PM+2/PM) increased significantly in treated BHT and 4-MP (where PM- primary molecule, PM+1- isotopic molecule either for 13C or 2H and PM+2 is the isotopic molecule for 18O). The isotopic abundance ratio of (PM+1/PM) in the treated BHT and 4-MP was increased up to 181.27% and 380.73% respectively as compared to their respective control. Moreover, the isotopic abundance ratio of (PM+2/PM) in the treated BHT and 4-MP increased up to 185.99% and 355.33% respectively. GC-MS data suggests that the biofield treatment significantly increased the isotopic abundance of 2H, 13C and 18O in the treated BHT and 4-MP as compared to the control.