Application of Separation Techniques in Novel Coronavirus (COVID-19) Research
Separation Techniques like liquid chromatography is used for purification of many different biomolecules. After a chromatographic separation, the fractions containing the target molecules are identified with specific detection. These fractions with the desired molecules are collected and used for next process steps. RNA purification with the help of chromatography is done at higher pressure and temperatures. Fast Protein Liquid Chromatography (FPLC) is a standard method in research with proteins for many years. With the help of FPLC the structure and activity of the isolated proteins can be investigated. The therapeutic potential of mRNA is extensively discussed in times of search for vaccines in the research of COVID-19. The idea is to provide the human body with mRNA, encoding therapeutic proteins, which the immune system uses to fight the virus. RNA can be produced and applied much easier compared to the full-length therapeutic proteins it is encoding for. In order to generate pure mRNA to administer, it was found that purification by chromatographic means is highly important. Research on virus specific proteins focusses for example on structural biology in order to analyze the structure of biomolecules and how alterations in their structures affect their function. Understanding the protein function on the molecular level gives valuable information for drug and vaccine development. The Journal of Chromatography and Separation Techniques aims to publish such novel work on chromatography/separation techniques which aids in COVID-19 Research.