Vitamin E metabolite 13-carboxychromanols inhibit pro-inflammator | 54258
Journal of Clinical and Cellular Immunology

Journal of Clinical and Cellular Immunology
Open Access

ISSN: 2155-9899

Vitamin E metabolite 13-carboxychromanols inhibit pro-inflammatory enzymes, induce apoptosis and autophagy in human cancer cells by modulating sphingolipids and suppress colon tumor development in mice

6th International Conference and Expo on Immunology

October 24-26, 2016 Chicago, USA

Qing Jiang

Purdue University, USA

Scientific Tracks Abstracts: J Clin Cell Immunol

Abstract :

Vitamin E forms are substantially metabolized to various carboxychromanols including 13â�?�?-carboxychromanols (13â�?�?-COOHs) that are found at high levels in feces. However, there is limited knowledge about functions of these metabolites. Here we studied �?´T-13â�?�?-COOH and �?´TE-13â�?�?-COOH, which are metabolites of �?´-tocopherol and �?´-tocotrienol, respectively. Both 13â�?�?-COOHs are much stronger than tocopherols in inhibition of pro-inflammatory and cancer promoting cyclooxygenase-2 (COX-2) and 5-lipoxygnease (5-LOX) and in induction of apoptosis and autophagy in colon cancer cells. The anti-cancer effects by 13â�?�?-COOHs appeared to be partially independent of inhibition of COX-2/5-LOX. Using liquid chromatography tandem mass spectrometry, we found that 13â�?�?-COOHs increased intracellular dihydrosphingosin and dihydroceramides after short-time incubation in HCT-116 cells and enhanced ceramides while decreased sphingomyelins during prolonged treatment. Modulation of sphingolipids by 13â�?�?-COOHs was observed prior to or coinciding with biochemical manifestation of cell death. Pharmaceutically blocking the increase of these sphingolipids partially counteracted 13â�?�?-COOH-induced cell death. Further, 13â�?�?-COOH inhibited dihydroceramide desaturase without affecting the protein expression. In agreement with these mechanistic findings, �?´TE-13â�?�?-COOH significantly suppressed the growth and multiplicity of colon tumor in mice. Our study demonstrates that 13â�?�?-COOHs have anti-inflammatory and anticancer activities may contribute to in vivo anticancer effect of vitamin E forms and are promising novel cancer prevention agents.

Biography :

Qing Jiang is a Professor in Nutrition Science at Purdue. She has focused on different forms of vitamin E and novel vitamin E metabolite long-chain carboxychromanols with respect to their anti-inflammatory and anticancer activities in cell-based and preclinical studies. Her lab has developed various analytical methods for quantifying vitamin E metabolites. Dr. Jiang has 45 publications and obtained three patents. She is a member of the editorial board of Journal of Nutritional Biochemistry. She has served as a reviewer in study sections of NIH and USDA. She is a recipient of E.L.R. Stokstad Award for outstanding fundamental research in nutrition from American Society for Nutrition and University Faculty Scholar Award from Purdue.