Virology & Mycology
Open Access
ISSN: 2161-0517
+44 1223 790975
ISSN: 2161-0517
+44 1223 790975
Naihui Chu and Wenjuan Nie
Beijing Chest Hospital, China
Posters & Accepted Abstracts: Virol Mycol
According to tuberculosis diagnosis, especially culture negative tuberculosis, pathology is the most important diagnostic method. Molecular pathology is a new method combining pathology and molecular biology method which improves sensitivity and specificity. In our hospital, IS6110 gene fragment was chosen as the target gene of tuberculosis molecular pathology. IS6110 has been found in M. tuberculosis and M. bovis but not in any of the other mycobacteria tested. Therefore, IS6110 will be used as a specific probe for the identification of the M. tuberculosis complex. Moreover, M. tuberculosis, contain multiple copies of IS6110, therefore use of this target should result in enhanced sensitivity. Molecular pathology by IS6110 was used in our hospital as a diagnostic method for 1 year. Dozens of patients were diagnosed successfully, which showed good sensitivity and specificity. However, sample of molecular biotechnology regularly confines to sputum or body fluid samples. In addition, diagnosis evidence of pathology only focuses on histomorphology and pathogen investigation. Both have low sensitivity and specificity. Molecular biotechnology leads pathology to a macrobiomolecular level, which has better sensitivity and specificity than histomorphology. The usage of molecular pathology on tuberculosis diagnosis should be popularized.
Email: chunaihui1994@sina.com