Virology & Mycology
Open Access

Titer on chip: New analytical tool for influenza vaccine potency determination

International Conference on Flu

June 08-10, 2015 Chicago, USA

Kathy Rowlen

Posters-Accepted Abstracts: Virol-mycol

Abstract :

Titer on Chip (Flu-ToC) is a new technique for quantification of influenza hemagglutinin (HA) concentration. In order to evaluate the potential of this new technique, a comparison of Flu-ToC to more conventional methods was conducted using recombinant HA produced in a baculovirus expression system as a test case. Samples from current vaccine strains were collected from four different steps in the manufacturing process. A total of 19 samples were analyzed by Flu-ToC (blinded), single radial immunodiffusion (SRID), an enzyme-linked immunosorbent assay (ELISA) and the purity adjusted bicinchoninic acid assay (paBCA). The results indicated reasonable linear correlation between Flu-ToC and SRID, ELISA and paBCA with regression slopes of log-log plots being 0.91, 1.03 and 0.91 respectively. The average ratio for HA content measured by Flu-ToC relative to SRID, ELISA and paBCA was 83%, 147% and 81%, respectively indicating nearly equivalent potency determination for Flu-ToC relative to SRID and paBCA. These results, combined with demonstrated multiplexed analysis of all components within a quadrivalent formulation and robust response to HA strains over a wide time period support the conclusion that Flu- ToC can be used as a reliable and time-saving alternative potency assay for influenza vaccines.