Virology & Mycology
Open Access

The assessment of equine herpesvirus type 1 (EHV-1) replication in equine dermal cells stimulated with synthetic TLR ligands

Joint Event 10^th International Virology Summit & 4^th International Conference on Influenza & Zoonotic Diseases

July 02-04, 2018 | Vienna, Austria

Golke A, Brzezicka J, Chodkowski M, Serafinska I, Cymerys J and Banbura M W

Warsaw University of Life Sciences, Poland

Posters & Accepted Abstracts: Virol Mycol

Abstract :

The body's ability to fight viral infection depends on the effective activation of the innate immune response. Activation of specific pattern recognition receptors (PRRs), including toll-like receptors (TLRs) triggers the production of proinflammatory cytokines, including interferons, which should lead to inhibition of viral replication. Because viruses have developed a number of mechanisms to interfere with TLR signaling, synthetic TLR activators can be considered as potential antiviral drugs. In the present study we have been investigating the effect of TLR synthetic ligands on the replication of equine herpesvirus type 1 (EHV-1) in ED cell line (equine dermal). Two different TLR ligands were used: TLR2/TLR6 ligand - Pam2CSK4 (0.1-100 �?¼g/ml) and TLR9 ligand - ODN D-SL03 (1-10 �?¼M/ml). The effect of TLR ligands on EHV-1 replication was assessed basing on the cytopathic effect and the quantitative evaluation of the number of viral DNA copies in the cells and the culture medium (real-time PCR). Both, on the basis of the cytopathic effect evaluation and the number of viral DNA copies quantification the antiviral activity of Pam2CSK4 at concentrations 10 �?¼g/ml and 100 �?¼g/ml was observed. No significant effect of ODN D-SL03 on EHV-1 replication was observed. However, preliminary observations based on the cytopathic effect evaluation showed that Pam2CSK4 at a concentration of 100 �?¼g/ml in combination with ODN D-SL03 at a concentration of 10 �?¼M/ml inhibited EHV-1 replication more efficiently than each of them individually. This stays with agreement with other studies, which show that it is necessary to stimulate both, TLR2 and TLR9 to trigger an effective immune response against herpes viral infections. Recent Publications
1. Duggan J M, You D, Cleaver J O, Larson D T, Garza R J, Guzm�?¡n Pruneda F A, Tuvim M J, Zhang J, Dickey B F and Evans S E (2011) Synergistic interactions of TLR2/6 and TLR9 induce a high level of resistance to lung infection in mice. Journal of Immunology 186:5916-5926.
2. Tuvim M J, Gilbert B E, Dickey B F and Evans S E (2012) Synergistic TLR2/6 and TLR9 activation protects mice against lethal influenza pneumonia. PLOS One 7:e30596.
3. Lima G K1, Zolini G P, Mansur D S, Freire Lima B H, Wischhoff U, Astigarraga R G, Dias M F, das Gra�?§as Almeida Silva M, B�?©la S R, do Valle Antonelli L R, Arantes R M, Gazzinelli R T, B�?¡fica A, Kroon E G and Campos M A (2010) Toll-like receptor (TLR) 2 and TLR9 expressed in trigeminal ganglia are critical to viral control during herpes simplex virus 1 infection. The American Journal of Pathology 177:2433-2445.

Biography :

Golke A is an Associate Professor in the Department of Preclinical Science, Faculty of Veterinary Medicine, Warsaw University of Life Sciences. Her scientific interests include neuroinfections and the innate immune response to viral infections, in particular, the use of PRRs ligands in therapy of infectious diseases.

E-mail: anna_golke@sggw.pl