Journal of Clinical Trials
Open Access

Synthesis and biological evaluation of pyridine derivatives as antimicrobial agents

6^th World Pharmacists and Clinical Pharmacy Annual Congress

May 22-23, 2017 Chicago, USA

Hussen Dagim Ali, Adnan A Bekhit and Ariaya Hymete

Mekelle University, Ethiopia
University of Alexandria, Egypt
Addis Ababa University, Ethiopia

Scientific Tracks Abstracts: J Clin Trials

Abstract :

In this study, several pyridine derivatives were synthesized and evaluated for their in vitro antimicrobial activity against Gram-positive bacteria (S. aureus and B. cereus), Gram-negative bacteria (P. aeruginosa and E. coli) and fungus (C. albican and A. niger). The intermediate chalcone derivative was synthesized by condensation of pyrazole aldehydes with acetophenone in alcoholic KOH. Cylization of chalcone derivative with ethyl cyanoacetate and ammonium acetate resulted in pyridine carbonitrile derivatives. Furthermore, condensation of pyridine-4-carboxaldeyhe with different amino-derivatives gave rise to pyridine derivatives. The oxadiazole derivative was prepared by cylization of hydrazone containing pyridines with acetic anhydride. Characterization of the synthesized compound was performed using IR, 1H NMR, 13C NMR spectra and elemental microanalyses. The antimicrobial results revealed that compounds hydrazone containing pyridines and oxadiazoles exhibited half fold antibacterial activity compared to ampicillin, against B. cereus. On the other hand, compound chlorinated pyridine carbonitril derivative showed an equivalent activity compared to miconazole against Candida albican (CANDAL 03) and to clotrimazole against the clinical isolate Candida albican 6647. Moreover, this compound chlorinated pyridine carbonitril derivative was further tested for its acute toxicity profile. The results showed that oral LD50 is more that 300 mg/ kg and parentral LD50 is more than 100 mg/kg. Compound chlorinated pyridine carbonitril derivative is a good candidate for antifungal agent with good toxicity profile and deserves more chemical derivatization and clinical study.

Biography :

Hussen Dagim Ali is a Pharmacist and Medicinal Chemist working at Mekelle University, College of Health Sciences and Ayder Comprehensive Specialized Hospital as an Assistant Professor. He has an immense experience in academic, administration, research and community services. Though he is previously involved in drug discovery researches, currently he is more focusing on improving health of community through identifying gaps in the health care delivery.

Email: dagim.ali@mu.edu.et