GET THE APP

Survey of causative agents for acute respiratory infections among | 3329
Virology & Mycology

Virology & Mycology
Open Access

ISSN: 2161-0517

+44 1223 790975

Survey of causative agents for acute respiratory infections among patients in Khartoum- State, Sudan, 2010-2011


International Conference on Flu

June 08-10, 2015 Chicago, USA

Khalid A Enan

ScientificTracksAbstracts-Workshop: Virol-mycol

Abstract :

Objective: This study was carried out to determine causative agents of acute respiratory illness of patients in Khartoum State, Sudan. Methods: Four hundred patients experiencing respiratory infections within January-March 2010 and January-March 2011 were admitted at Khartoum Hospital and had their throat swab samples subjected to multiplex real-time RT-PCR to detect influenza viruses (including subtypes) and other viral agents. Isolation, nucleotide sequence and phylogenetic analysis on some influenza viruses based on the HA gene were done. Results: Out of 400 patients, 66 were found to have influenza viruses (35, 27, 2, and 2 with types A, B, C, and A and B coinfections, respectively). Influenza viruses were detected in 28, 33 and 5 patients in the age groups <1, 1�??10, and 11�??30 years old, respectively but none in the 31�?? 50 years old group. Out of 334 patients negative for influenza viruses, 27, 14, and 2 were positive for human respiratory syncytial virus, rhinovirus and adenovirus, respectively. Phylogenetic tree on influenza A (H1N1)pdm09 subtype shows that Sudan strains belong to the same clade and are related to those strains from several countries such as USA, Japan, Italy, United Kingdom, Germany, Russia, Greece, Denmark, Taiwan, Turkey and Kenya. Seasonal A H3 subtypes have close similarity to strains from Singapore, Brazil, Canada, Denmark, USA and Nicaragua. For influenza B, Sudan strains belong to two different clades, and just like influenza A (H1N1)pdm09 and A H3 subtypes, seem to be part of worldwide endemic population (Kenya, USA, Brazil, Russia, Taiwan and Singapore). Conclusions: In Sudan, the existence of respiratory viruses in patients with acute respiratory infection was confirmed and characterized for the first time by using molecular techniques.

Biography :

Khalid A Enan has completed his PhD at the age of 34 years from Sudan Academy of Sciences. He is the Head of Department of Virology, Central Laboratory and Co-ordinator of Scientific Committee Central Laboratory. He is a researcher, diagnoses of viral diseases using different serological testes, cell culture, and molecular diagnostic tools such as PCR, Real-time PCR and LAMP PCR. He guides Master and PhD students in their research and participates in the practical sessions of virology training courses in the lab. He works in the Virology Unit and participates in the research projects running in the Lab. He has published more than 21 papers in reputed journals and is a Member of the Board of the Department of Microbiology, Faculty of Medical Laboratory Sciences- El Nellin University.

Top