Virology & Mycology
Open Access

Rapid and accurate identification of clinically significant fungi by molecular methods

International Conference on Mycology & Mushrooms

September 12-14, 2016 San Antonio, USA

Wieslaw Furmaga

University of Texas Health Science Center at San Antonio, USA

Posters & Accepted Abstracts: Virol Mycol

Abstract :

Rapid and accurate identification (ID) of clinically significant fungi is essential and DNA sequencing of specific gene has become an important tool for fungal molecular ID. The molecular methods are used to solve varies diagnostic dilemma related to the fungi infection such as: Sensitivity and accuracy of testing: Although the internal transcribed spacer (ITS) and domains 1 and 2 of the large ribosomal subunit (D1/D2) are recommended for fungi identification by the CLSI guideline MM18-A, these targets may not be sufficient to discriminate between certain species. For example, the identification of Aspergillus, a clinically important species in immunocompromised patients may be enhanced by sequencing of the beta tubulin and calmodulin targeting regions. Similarly Fusarium species and potential human pathogen, causing local or disseminated infections in immunocompromised patients can be identify by Sanger sequencing of ITS, translation elongation factor and RNA polymerase II second largest subunit. Distinction pathogenic from non-pathogenic organisms R. arrhizus causes a local infection of the lungs, brain, etc., and severe systemic mycosis. Closely related and morphologically indistinguishable R. Delemar may have a different pathogenesis and antifungal susceptibility. They can be separated by ITS sequence. Drug susceptibility: Azole antifungals (itraconazole, voriconazole, posaconazole and isavuconazole) are effective treatments for invasive aspergillosis. However, drug resistance in A. fumigatus has been reported and can be identified by a testing for cyp51A missense mutations. Sanger sequencing is an effective supportive diagnostic method for fungi identification, differentiation between pathogenic and non-pathogenic organisms and for assessment of susceptibility to the anti-fungus treatment.

Biography :

Email: furmaga@uthscsa.edu