Ahmed Mohammed Alamin Ahmed Elhadi
Al Neelain University, Sudan
Posters & Accepted Abstracts: Virol-mycol
Novel DNA virus named Torque Teno Virus (TTV) was isolated in 1997 from the sera of patients with post transfusion non A-G hepatitis (TTV) has been reported in world with high prevalence. This study investigates the frequency of TTV viremia in 83 blood donors in Khartoum state, Sudan. Serum samples were screened for TTV DNA using polymerase chain reaction (PCR) to detected two regions, untranslated region (UTR) and (N22) region. Fifty one (61%) out of 83 healthy blood donors and 68 (83%) out of 81 healthy blood donors were shown to be positive for TTV using nested PCR to detect N22 region and single step PCR to detect UTR region respectively. The results which are statistically analyzed show that there was no significant differences in DNA prevalence of (TTV) among blood donors (P>0.05) in their serum samples. This study is comparable with other healthy population in Africa and in developed countries around the world. The present study is one of first attempts to reports directly the prevalence rate of TTV in the studied group and focus on the molecular diagnosis of TTV. It revealed that TTV was circulating in Sudan among blood donors. The results of present study demonstrated a high prevalence of TTV in studied group. Further studies are required to determine prevalence of TTV in other groups.