Rodrigo Pinheiro Araldi
Butantan Institute, Brazil
Posters & Accepted Abstracts: Virol-mycol
Bovine papillomavirus (BPV) is considered a useful model for HPV oncogenic process study. Both BPV and HPV are recognized as able to promoter cancer initiation and progression. Our results have reinforced the BPV mutagenic potential, which contributes to genomic instability and cancer initiation. However, currently results also point out that BPV promotes metabolic changes in host cells which induce Warburg effect after malignant transformation. This action is discussed as a consequence of hyper proliferative action of viral oncoproteins (E5, E6 and E7). This effect reduces the reactive oxygen species (ROS) production which is associated to cell survival. Moreover, both papilloma and esophageal carcinoma cell lines coinfected by BPV-1, 2 and 4 showed a migratory phenotype which was verified by the presence of lamellipodia and filopodia using immunofluorescence (IF) and time-lapse microscope. These data are evidences that BPV induces epithelial-mesenchymal transition (EMT), process characterized by the apoptosis resistance, metabolic changes and migratory phenotype acquisition. In order to verify the presence of EMT in these cells, expression levels of epithelial and mesenchymal proteins were evaluated by IF and flow cytometry. Results pointed out a reduction of epithelial biomarker expression levels which were accompanied by an increase of mesenchymal proteins. Thus, the combinatory results bring strong evidences that BPV participates of cancer initiation, progression and metastasis. Due to the morphological, genomic and pathological similarities between BPV and HPV, these results have to be considered in human infections.