Transcriptomics: Open Access
Open Access

Multicomponent hybridization probes for nucleic acid analysis

Global Congress on Nucleic Acids: Biology, Health & Diseases

August 04-05, 2016 New Orleans, USA

Dmitry M Kolpashchikov

University of Central Florida, USA

Posters & Accepted Abstracts: Transcriptomics

Abstract :

Hybridization of nucleic acid probes to DNA or RNA targets remains one of the most common strategy in nucleic acid analysis. Formats that use hybridization probes include rtPCR, microarrays, fluorescent in situ hybridization (FISH) to name a few. Instead of the traditional single stranded probes, we develop multicomponent hybridization probes (MHP). In MHP approach, several oligonucleotide strands cooperatively associate with a target DNA or RNA sequence and produce detectable signal. The MHP developed by us recently include split broccoli and split spinach aptamers, antenna tile-associated deoxyribozyme sensor, molecular beacon sensors among others. The sophisticated design of MHP enables improving selectivity, limit of detection and cost efficiency. Moreover, it allows detection of folded RNA at low temperatures and simplifies probe design in some cases.

Biography :

Email: dmitry.kolpashchikov@ucf.edu