Transcriptomics: Open Access
Open Access

Mapping differential gene expression of vancomycin resistant Enterococci associated to Eicchornia crassipes roots

International Conference on Transcriptomics

July 27-29, 2015 Orlando, USA

Pushpa Lata1 and Rishi Shanker2

Accepted Abstracts: Transcriptomics

Abstract :

Enterococci serve as an �??indicator�?� of fecal contamination for recreational water quality. The vancomycin resistant Enterococci (VRE) are emerging environmental contaminants in the surface waters. We have earlier reported higher concentration of VanA gene associated to an aquatic macrophyte Eicchornia crassipes (Mart.) Solms, than free flowing river Gomti water. In this study we investigated the differential gene expression off srABDC, gelE and sprE involved in biofilm formation along with vanA and vanB genes. Results of SYBR green based reverse transcriptase quantitative PCR indicates 4-10 fold up-regulation of the fsrABDC, gelE, sprE, vanA and vanB genes in the microbiota associated to E. crassipes root-mats as compared to river water (Student�??s t test p<0.001). Our study suggests, the aquatic macrophytes like E. crassipes growing in polluted river waters can act as secondary reservoir and nonpoint source of lethal biofilm forming VRE in freshwater bodies. The biofilm forming VRE infection has been reported in literature asdifficult medical condition. So, the aquatic macrophytes sustaining higher concentrations of VRE superbugs can pose a serious threat to public health as in India a large population gets exposed to river water for daily needs.