Massimo De Paschale
Scientific Tracks Abstracts: JAA
In serological diagnosis of mononucleosis EBV infection it is usually easy to distinguish between acute or past infections: the presence of VCA IgG and IgM without EBNA-1 IgG indicates acute infection, while the presence of VCA IgG and EBNA-1 IgG without VCA IgM is typical of past infection. However, there can be serological patterns diffi cult to interpret. For example a pattern of VCA IgG isolated (without VCA IgM and EBNA-1 IgG) that can be present both in case of acute or past infection, requires other laboratory tests, such as VCA IgG avidity, Immunoblotting for EBV IgG , the research of heterophile antibodies, anti-EA antibodies or viral genomes with genic amplifi cation techniques. Th ese tests can defi ne the status of the infection without having a second sample at a distance of time, which may be requested in the hope of an antibody change. Also a profi le of simultaneous presence of VCA IgG, VCA IgM and EBNA-1 IgG gives rise to some interpretative doubts because occurring both in the case of recent infection and in case of reactivation and requires other laboratory tests. Th e choice of these additional tests depends on various factors beyond the scientifi c and technical appropriateness. Surely organizational and economic reasons infl uence the choice, but also the number of samples with inconclusive serological results in routine and the type of patients (immunocompetent or immunocompromised patients) can direct the choice of additional tests.
Graduated with honors in Biology at State University of Milan (Italy), Postgraduate Diploma with honors in Hygiene at State University of Milan (Italy), Postgraduate Diploma with honors in Clinical Pathology at State University of Pavia (Italy), Certifi cat d?Université d?Enseignement Européen de Transfusion Sanguine at Université Louis Pasteur de Strasbourg (France), Postgratuate Diploma with honors in Microbiology and Virology at State University of Milan (Italy). Currently Head of Serology Laboratory of Microbiology Unit at Legnano Hospital (Italy). Author and/or co-author of 36 papers in National and International Journals and of more than 90 communications to national and international conferences.