Isobaric tags for relative and absolute quantitation labelling-ba | 12278
Virology & Mycology

Virology & Mycology
Open Access

ISSN: 2161-0517

+44 1223 790975

Isobaric tags for relative and absolute quantitation labelling-based proteomic analysis of Mycoplasma mycoides, causative agent of contagious bovine pleuropneumonia

Joint Event 10th International Virology Summit & 4th International Conference on Influenza & Zoonotic Diseases

July 02-04, 2018 | Vienna, Austria

Haili Li, Yindi Xu, Zhifang Wang, Wenhao Zhu, Qingxian Zhang, Jianyu Fang, Wenqiang Jiao, Yi You, Limin Lang, Lixian Zhang, Keling Wang and Zihua Hou

Henan Academy of Agricultural Sciences-CAAS, China

Posters & Accepted Abstracts: Virol Mycol

Abstract :

Contagious bovine pleuropneumonia (CBPP), an endemic disease, is still threatening cattle health. A Proteomics study of mycoplasma mycoides is rarely known. This study screened the differentially expressed proteins (DEPs) between standard strains (PG45) and field isolate strains (HN12, Henan Province, China) using isobaric tags for relative and absolute quantitation labeling (iTRAQ). A total of 447 proteins were identified, 66 DEPs were quantified, 25 of which were up-regulated and 41 down-regulated between the two strains. Bioinformatic analysis revealed that these DEPs are involved in a wide variety of biology process played roles in Mycoplasma mycoides. Functional and KEGG pathway analysis revealed that the differential identified protein were related to bacterial secretion system, ABC transporters, HIF-1 signaling pathway, protein export, RNA degradation, mismatch repair, DNA replication, homologous recombination, aminoacyl-tRNA biosynthesis, ribosome, tyrosine metabolism, pentose phosphate pathway, glycolysis/gluconeogenesis, carbon fixation in photosynthetic organisms, oxidative phosphorylation, photosynthesis. This is the first comparative proteomic report on standard strains (PG45) and field isolate strains. These identified protein data will provide new view valuable information for understanding the molecular mechanisms of Mycoplasma mycoides. And these proteins may potentially act as bio-markers for diagnosis, vaccine or predict developmental competence of Mycoplasma mycoides.