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Impact of hepatitis-C virus infection on neutrophil oxidative burst function in hemodialysis patients
Virology & Mycology

Virology & Mycology
Open Access

ISSN: 2161-0517

Impact of hepatitis-C virus infection on neutrophil oxidative burst function in hemodialysis patients


6th Euro Virology Congress and Expo

March 10-12, 2016 Madrid, Spain

Gehan Saddik Abd El Hamid El Hadidy, Hassoba H M, Younis S E, Fahmy H A, Labib S, Abdelrazek N Y, Youssef A and Metwally L

Suez Canal University, Egypt

Posters & Accepted Abstracts: Virol-mycol

Abstract :

Polymorphonuclear leukocyte (PMN) functions have been studied extensively in hemodialysis (HD) patients; however, results are contradictory and the mechanisms that modulate phagocytosis and oxidative burst are not completely understood. Hepatitis-C virus (HCV) is a frequent complication of HD that may be associated with disturbed PMN function; however, the impact of HCV infection on neutrophil oxidative burst function in HD patients is unknown. We investigated neutrophil oxidative burst function in 24 HD patients (15 HCV-positive and 9 HCV-negative patients) before and after dialysis. HCV-RNA was detected by RT-nested PCR while, quantitative measurement of oxidative burst function was assessed by flow cytometry. Neutrophil oxidized burst function was significantly diminished in HD patients as compared to controls (P=0.001, oxidized PMN (%); P=0.02 mean fluorescence intensity, MFI) and in pre-dialysis as compared to post-dialysis samples (oxidized PMNs (%): 60.5?±3.2 vs. 72.1?±3.9, P=0.02); (MFI: 352?±42 vs. 500?±50, P=0.03). Alteration in neutrophil oxidative burst function in the pre-dialysis samples was significant in HCV-positive patients as compared to HCV-negative patients (oxidized PMNs (%): 50?±2.9 vs. 63?±5.1, P=0.02); (MFI: 291?±31 vs. 438?±64, P=0.006). Marked reduction in E. coli induced burst in pre-dialysis samples compared to post-dialysis was found in HCVpositive when compared to HCV-negative patients (oxidized PMNs (%): 50?±2.9 vs. 74.8?±4.7, P=0.001), (MFI: 291?±31 vs. 493?±63, P=0.002). In conclusion, a possible role of concomitant HCV infection in alteration of neutrophil oxidative burst function is highly suggested.

Biography :

Gehan Saddik Abd El Hamid El Hadidy has completed her MD from Suez Canal University, Egypt. She is presently working as a Professor of Medical Microbiology and Immunology in Faculty of Medicine, Suez Canal University. She has published around 9 papers in reputed journals.

Email: [email protected]

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