Virology & Mycology
Open Access
ISSN: 2161-0517
+44 1223 790975
ISSN: 2161-0517
+44 1223 790975
Patricia Filippsen Favaro, Paulo E Brand�?£o1, Eliana M C Villalobos, Maria C C S H Lara, Elenice M S Cunha, Pollyana R C Braga, Enio Mori and Leonardo J Richtzenhain
University of S�?£o Paulo, Brazil
Biological Institute, Brazil
Fort Dodge/Pfizer Animal Health, Campina, SP, Brazil
Pasteur Institute, Brazil
Posters & Accepted Abstracts: Virol Mycol
The equine influenza virus (EIV) is responsible for one of the most important respiratory diseases in horses, mules and donkeys. Three H3N8 virus strains were isolated from horses with respiratory disease in the State of S�?£o Paulo during the Brazilian outbreak in 2012. Viral RNA was extracted from the allantoic fluid and cDNA synthesized. Amplification and sequencing were applied to the HA gene of the three strains, while the eight genes of A/equine/Sao Paulo/1.19/2012 were sequenced and analyzed. Specific primers were applied for amplification and sequencing. Polymerase (PA, PB1 and PB2) internal primers were designed for sequencing so as to allow complete codon analysis. The confidence levels of all the sequences were evaluated using the FinchTVâ�?¢ software. The eight genes from different EIVs available in the GenBank and EpiFlu (GISAID) databases were compared with the Sao Paulo/1.19/2012 strain. The nt neighbor-joining trees were constructed using Mega 5.0.5, by means of the Maximum Composite Likelihood substitution model and Bootstrap method for phylogeny test, with 1,000 repetitions. The nucleotide sequences were deposited in GenBank (accession numbers: KJ955628, KM032366 - KM032369 and KM190933 - KM190935, KT460192 and KT460193). The hemagglutinin was most identical to the Rio Grande do Sul/2012, Dubai/2012, Argentina/2012 and Uruguay/2012 strains and to some North American/2011-2012 strains. All eight genes clustered in the Florida Clade 1 sublineage. This study provides genomic information about EIV Brazilian strains from State of S�?£o Paulo.
Recent Publications:
1. Favaro P F, et al (2017). Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015. Brazilian Journal of Microbiology. (16) 31368.
3. Hoffmann E, et al (2001). Universal primer set for the full-length amplification of all influenza A viruses. Ach. Virol. 146 (12) 2275-2289.
4. Tamura K, et al (2011). Mega 5: molecular molecular evolutionary genetics analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony methods. Mol. Biol. Evol. 28(10) 2731-2739.
5. Felsenstein J (1984): Phylogenies and the comparative method. Am. Nat. 125, 1-15.
Patricia Filippsen Favaro has completed her Masters and PhD in veterinary science at Universidade de São Paulo. She has been the Visiting Scholar at Maxwell H Gluck Equine Research Center, the OIE Reference Laboratory for Equine Influenza. She has proficiency in molecular biology, diagnostics of infectious diseases, scientific writing, scientific presentations and meetings. She has strong organizational and analytical skills, critical thinking, confidence and ability to work with hierarchical levels.
E-mail: patriciafilippsen@gmail.com